CURIO SEEKER SPATIAL TRANSCRIPTOMICS KITS

High-resolution spatial transcriptomics made simple

Curio Seeker Spatial Transcriptomics Kits enable whole transcriptome, spatial mapping of fresh frozen tissues at industry-leading resolution, and are compatible with your favorite eukaryotic organism. The assay plugs directly into existing sequencing workflows and requires no new specialized instrumentation or novel probe sets.  Begin your spatial discovery today.

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Product highlights

Keep your focus with the Curio Seeker 3×3 kit or expand your horizons with the 10×10 version.

Single-cell resolution

A tightly-packed monolayer of beads delivers a continuous view of your region of interest at 10 μm resolution.

Whole transcriptome

Whole transcriptome analysis that is not limited by predetermined targets or throughput, enabling true unbiased discovery.

No specialized instrumentation

Leverages standard molecular biology techniques and instrumentation; no new specialized instrumentation or microscopy expertise needed.

Flexible across organisms and tissues

Analyze any poly adenylated RNA across a wide array of organisms and tissue types.

Sized to meet your needs

Conveniently offered in 3 mm x 3 mm or 10 mm x 10 mm tile sizes without sacrificing resolution.

Fast time to results

Multiple tissue samples can be prepared for sequencing in just one day.

Curio Seeker tile

The Curio Seeker tile preserves spatial information of the tissue transcriptome by capturing RNA on a monolayer of tightly-packed, spatially-indexed, 10 μm beads. 

Simple, single-day workflow without specialized instrumentation

Once your tissue section is placed on the Curio Seeker tile, mRNA is captured and hybridized on the spatially-indexed beads before reverse transcription. The indexed beads are then dissociated from the tile and the tissue digested, followed by second strand synthesis and cDNA amplification. Purified cDNA undergoes NGS library preparation and is sequenced. The resulting FASTQ files are processed by the Curio Seeker bioinformatics pipeline to create a continuous whole transcriptome spatial map of your tissue section.

Accelerate discoveries with high-resolution,
whole transcriptome expression maps

The Curio Seeker tile captures any poly adenylated nucleic acid molecules, resulting in an unbiased, whole transcriptome spatial gene expression map. No pre-selection of gene targets nor generation of gene panels are required.

Mouse embryo (E11) analyzed with Curio Seeker 10×10 kit. Spatial gene expression patterns of the top 10 spatially variable genes are shown.

Amenable to a wide variety of organisms and tissues

Generate high-resolution transcriptomics information across a wide range of tissue types and high-order organisms using the same protocol.

Go beyond mouse, rat, and human tissues – researchers have successfully applied Curio Seeker to tissues from primates, chicken, worms, seeds from plants, and beyond.

Mouse liver
Mouse spleen
Mouse kidney

Sized to meet your tissue samples
and research needs

3x3 output

10x10 output

Example Curio Seeker results from mouse brain sections.

Sequencing libraries
in approximately 8.5 hours

The Curio Seeker spatial mapping kit includes 8 (3 mm x 3 mm) or 4 (10 mm x 10 mm) tiles and reagents to prepare cDNA from your captured RNA. For indexing and multiplex sequencing, the Curio Seeker dual indexing primer kit is required and supports 40 (3 mm x 3 mm) or 5 (10 mm x 10 mm) Curio Seeker tiles. Together, these kits generate sequencing libraries in approximately 8.5 hours.

Features

Curio Seeker 3x3 kit

Curio Seeker 10x10 kit

Plex

Whole transcriptome

Resolution

10 μm

Special capital equipment

None required

Sample type

Fresh frozen

Analysis software Included

Curio Seeker bioinformatics pipeline

Tile size

3 mm x 3 mm

10 mm x 10 mm

Duration/hands-on time

8 hrs with 2.5 hrs hands-on time

8.5 hrs with 3 hrs hands-on time

Recommended sequencing depth

~ 400 M

~ 2 B

Features

Curio Seeker 3x3 kit

Plex

Whole transcriptome

Resolution

10 μm

Special capital equipment

None required

Sample type

Fresh frozen

Analysis software Included

Curio Seeker bioinformatics pipeline

Tile size

3 mm x 3 mm

Duration/hands-on time

8 hrs with 2.5 hrs hands-on time

Recommended sequencing depth

~ 400 M

Features

Curio Seeker 10x10 kit

Plex

Whole transcriptome

Resolution

10 μm

Special capital equipment

None required

Sample type

Fresh frozen

Analysis software Included

Curio Seeker bioinformatics pipeline

Tile size

10 mm x 10 mm

Duration/hands-on time

8.5 hrs with 3 hrs hands-on time

Recommended sequencing depth

~ 2 B

Proven technology

Check out the list of 35+ publications to see how researchers applied Curio Seeker and Slide-seq, the foundational technology behind Curio Seeker, across a wide varieties of organisms and tissues, and in research areas spanning neuroscience, developmental biology, immunology, oncology, plant biology, and beyond.

Ordering information

Curio Seeker 3x3 kit

Part number

3x3 Seeker Kit Bundle V1.1 (8 tiles)

SK004

Curio Seeker Dual Indexing Primer Kit V2

K006

Curio Seeker 10x10 kit

Part number

10x10 Seeker Kit Bundle (4 tiles)

SK003

Curio Seeker Dual Indexing Primer Kit V2

K006

Thermal Cycler Adapter

TJ002

Ordering information

Curio Seeker 3x3 kit

Part number

3x3 Seeker Kit Bundle V1.1 (8 tiles)

SK004

Curio Seeker Dual Indexing Primer Kit V2

K006

Curio Seeker 10x10 kit

Part number

10x10 Seeker Kit Bundle (4 tiles)

SK003

Curio Seeker Dual Indexing Primer Kit V2

K006

Thermal Cycler Adapter

TJ002

Learn more

Curio Seeker – Technology overview

Customer perspectives – Integrating into a core lab setting

Curious?

Explore Curio Seeker documentation

Read details of the Curio Seeker kits

Access an overview of the Curio Seeker technology

See the kind of data you can uncover with Curio Seeker kits