CURIO SEEKER SPATIAL MAPPING KITS

High-resolution spatial transcriptomics made simple

Curio Seeker Spatial Mapping Kits enable whole transcriptome, spatial mapping of fresh frozen tissue at industry-leading resolution and plugs directly into existing sequencing workflows.

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The Curio Seeker tile preserves spatial information of the tissue transcriptome by capturing RNA on a monolayer of tightly-packed, spatially-indexed, 10 μm beads. 

Once captured, the RNA undergoes reverse transcription, library preparation, and sequencing. The sequencing data is then reconstructed bioinformatically into a continuous, whole transcriptome map of your region of interest. 

Product highlights

Keep your focus with the Curio Seeker 3X3 kit or expand your horizons with the 10×10 version.

Single-cell resolution

A tightly-packed monolayer of beads delivers a continuous view of your region of interest at 10 μm resolution.

Whole transcriptome

Whole transcriptome analysis that is not limited by predetermined targets or throughput, enabling true unbiased discovery.

Simple workflow

Leverages standard molecular biology techniques and instrumentation; no new specialized instrumentation or microscopy expertise needed.

Flexible

Whole transcriptome analysis that is not limited by predetermined targets or throughput, enabling true unbiased discovery.

Sized to meet your needs

Conveniently offered in 3x3 or 10x10 tile sizes without sacrificing resolution.

Fast time to results

Multiple tissue samples can be prepared for sequencing in just one day.

Curio Seeker workflow

Once your tissue section is placed on the Curio Seeker tile, mRNA is captured and hybridized on the spatially-indexed beads before reverse transcription. The indexed beads are then dissociated from the tile and the tissue digested, followed by second strand synthesis and cDNA amplification. After purification and quantification, the cDNA undergoes NGS library preparation and is sequenced on an Illumina sequencer. Lastly, the resulting FASTQ files are processed by the Curio Seeker bioinformatics pipeline to create a detailed spatial transcriptomic map of your tissue section.

High-resolution
expression maps

Standard html output from the Curio Seeker bioinformatics pipeline includes results from unbiased clustering and dimension reduction as well as a spatial map of identified clusters within a mouse hippocampus (left, 3×3 tile) and whole mouse brain (right, 10×10 tile). These plots enable the end user to easily determine if the data is biologically meaningful and decide how to best move forward with their analysis.

The Curio Seeker bioinformatics pipeline maps the spatial gene expression within a murine hippocampus section (3 mm x 3 mm tile, top row) orwhole murine brain (10 mm x 10 mm tile, bottom row). The expression patterns of the top five spatially-variable genes are shown here. These images highlight the sharp spatial boundaries of gene expression that can be achieved on the Curio Seeker spatial mapping kit.
(click to enlarge)

3x3 output

10x10 output

Amenable to a wide variety of tissue types

Generate high-resolution transcriptomics information across a wide range of tissue types using the same protocol.

Mouse Liver (click to enlarge)

Mouse Spleen (click to enlarge)

Mouse Kidney (click to enlarge)

Sized to meet your tissue samples
and research needs

The Curio Seeker spatial mapping kit includes 8 (3 mm x 3 mm) or 4 (10 mm x 10 mm) tiles and reagents to prepare cDNA from your captured RNA. For indexing and multiplex sequencing, the Curio Seeker dual indexing primer kit is required and supports 40 (3 mm x 3 mm) or 5 (10 mm x 10 mm) Curio Seeker tiles. Together, these kits generate sequencing libraries in approximately 8.5 hours.

Features

Curio Seeker 3x3 kit

Curio Seeker 10x10 kit

Plex

Whole transcriptome

Resolution

10 μm

Special capital equipment

None required

Sample type

Fresh frozen

Analysis software Included

Curio Seeker bioinformatics pipeline

Tile size

3 mm x 3 mm

10 mm x 10 mm

Duration/hands-on time

8 hrs with 2.5 hrs hands-on time

8.5 hrs with 3 hrs hands-on time

Recommended sequencing depth

~ 400 M

~ 2 B

Features

Curio Seeker 3x3 kit

Plex

Whole transcriptome

Resolution

10 μm

Special capital equipment

None required

Sample type

Fresh frozen

Analysis software Included

Curio Seeker bioinformatics pipeline

Tile size

3 mm x 3 mm

Duration/hands-on time

8 hrs with 2.5 hrs hands-on time

Recommended sequencing depth

~ 400 M

Features

Curio Seeker 10x10 kit

Plex

Whole transcriptome

Resolution

10 μm

Special capital equipment

None required

Sample type

Fresh frozen

Analysis software Included

Curio Seeker bioinformatics pipeline

Tile size

10 mm x 10 mm

Duration/hands-on time

8.5 hrs with 3 hrs hands-on time

Recommended sequencing depth

~ 2 B

Ordering information

Curio Seeker 3x3 kit

Part number

3x3 Seeker Kit Bundle V1.1 (8 tiles)

SK004

Curio Seeker Dual Indexing Primer Kit V2

K006

Curio Seeker 10x10 kit

Part number

10x10 Seeker Kit Bundle (4 tiles)

SK003

Curio Seeker Dual Indexing Primer Kit V2

K006

Thermal Cycler Adapter

TJ002

Ordering information

Curio Seeker 3x3 kit

Part number

3x3 Seeker Kit Bundle V1.1 (8 tiles)

SK004

Curio Seeker Dual Indexing Primer Kit V2

K006

Curio Seeker 10x10 kit

Part number

10x10 Seeker Kit Bundle (4 tiles)

SK003

Curio Seeker Dual Indexing Primer Kit V2

K006

Thermal Cycler Adapter

TJ002

Learn more

Curio Seeker – Technology overview

Customer perspectives – Integrating into a core lab setting

Curious?

Explore Curio Seeker documentation

Read details of the Curio Seeker kits

Access an overview of the Curio Seeker technology

See the kind of data you can uncover with Curio Seeker kits