CURIO SEEKER SPATIAL MAPPING KITS
Curio Seeker spatial mapping kits enable whole transcriptome, spatial mapping of fresh frozen tissue at industry-leading resolution and plugs directly into existing sequencing workflows.
The Curio Seeker tile preserves spatial information of the tissue transcriptome by capturing RNA on a monolayer of tightly-packed, spatially-indexed, 10 μm beads.
Once captured, the RNA undergoes reverse transcription, library preparation, and sequencing. The sequencing data is then reconstructed bioinformatically into a continuous, whole transcriptome map of your region of interest.
Keep your focus with the Curio Seeker 3X3 kit or expand your horizons with the 10×10 version.
Once your tissue section is placed on the Curio Seeker tile, mRNA is captured and hybridized on the spatially-indexed beads before reverse transcription. The indexed beads are then dissociated from the tile and the tissue digested, followed by second strand synthesis and cDNA amplification. After purification and quantification, the cDNA undergoes NGS library preparation and is sequenced on an Illumina sequencer. Lastly, the resulting FASTQ files are processed by the Curio Seeker bioinformatics pipeline to create a detailed spatial transcriptomic map of your tissue section.
Standard html output from the Curio Seeker bioinformatics pipeline includes results from unbiased clustering and dimension reduction as well as a spatial map of identified clusters within a mouse hippocampus (left, 3×3 tile) and whole mouse brain (right, 10×10 tile). These plots enable the end user to easily determine if the data is biologically meaningful and decide how to best move forward with their analysis.
The Curio Seeker bioinformatics pipeline maps the spatial gene expression within a murine hippocampus section (3 mm x 3 mm tile, top row) orwhole murine brain (10 mm x 10 mm tile, bottom row). The expression patterns of the top five spatially-variable genes are shown here. These images highlight the sharp spatial boundaries of gene expression that can be achieved on the Curio Seeker spatial mapping kit.
(click to enlarge)
The Curio Seeker spatial mapping kit includes 8 (3 mm x 3 mm) or 4 (10 mm x 10 mm) tiles and reagents to prepare cDNA from your captured RNA. For indexing and multiplex sequencing, the Curio Seeker dual indexing primer kit is required and supports 40 (3 mm x 3 mm) or 5 (10 mm x 10 mm) Curio Seeker tiles. Together, these kits generate sequencing libraries in approximately 8.5 hours.
Curio Seeker – Technology overview
Customer perspectives – Integrating into a core lab setting