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The Curio Seeker kit is the first high-resolution, whole-transcriptome, spatial mapping kit enabling researchers to analyze spatial differential gene expression of a region of interest.
No. You only need the tools found in a standard molecular biology lab such as heat blocks, thermal cyclers and access to an Illumina sequencer.
Whole transcriptome gene expression in a defined region of interest.
Fresh Frozen tissues.
3 mm x 3 mm
The spatial resolution is 10 micron.
Tissue sections for Curio Seeker Tile analysis should be 10 microns.
8 hours with 2.5 hours of hands-on time
Although we recommend 200 million paired-end reads for the murine hippocampus, deeper sequencing may be required for more transcriptionally active tissue types.
Sequenced Curio Seeker tile data can be analyzed with the Curio Seeker pipeline.
The pipeline reconstructs a spatial map of gene expression from FASTQ files generated by sequencing of the Curio Seeker libraries. It additionally performs quality control to help users determine next steps.
The environments that are compatible with the Curio Seeker pipeline are single-server linux station, HPC SLURM, HPC SGE, AWS.
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